Figure 5. HIF inhibition by Acriflavin restored LIG4 downregulation.

(A) HIF-1α and HIF-2α are upregulated in chronic hypoxia. U87-MG and D566-MG cells were incubated in 1% O₂ and 21% O₂ for 0–120 h. Protein was extracted and the levels of HIF-1α and HIF-2α determined by western blotting in two independent experiments. A representative blot is shown with quantification of the bands, normalised to T0 (B) D566‑MG and U87-MG cells were incubated in 21% and 1% O₂ for 24 h with and without 5 µM Acriflavin. LIG4 mRNA levels were measured by RT-PCR. Data are represented as log₂ fold change in mRNA levels with respect to 21% O₂ samples. Data are the mean of at least three independent experiments, with error bars showing S.E.M.