FIG 1.

Effect of storage on fecal microbiome composition with shallow shotgun metagenomic sequencing. (A) Beta-diversity of shotgun metagenomic sequencing data estimated with weighted UniFrac, colored by participants. Diamonds represent fresh samples. (B) Weighted UniFrac distances between stored samples and baseline samples (fresh, ambient) for each preservative. (C) Pearson correlation where each point represents a genus with the relative abundance in the fresh fecal samples on the x axis and the relative abundance in the stored fecal sample stored for 8 weeks on the y axis, with each plot series representing a different storage temperature. Both axes are presented in log base 10 scale. (D) Fraction of taxa with greater than 10-fold change relative to fresh samples for each storage condition.