FIG 1.

The Jag domain directs EloR to midcell. (A) Schematic representation of EloR, including predicted domains and domain borders. (B) Micrographs showing the subcellular localization of EloR-mKate2 (AW407), Jag-mKate2 (AW408), Jag-linker-mKate2 (AW409), and linker-mKate2 (AW410). Phase-contrast and corresponding fluorescence images are shown. The numbers above the micrographs indicate the amino acids (aa) of EloR utilized in the different constructs. The percentages of cells that displayed midcell localization of the mKate2 fusions are indicated, as are the numbers of cells included in the analyses. Bars, 2 μm. (C) Analysis of subcellular localization. For the strains where the majority of fusion proteins (EloR-mKate, Jag-mKate, and Jag-linker-mKate) displayed midcell localization, fluorescence maxima were detected and plotted in focus density plots using MicrobeJ (see Materials and Methods). x and y in the focus density plots denote the relative length and width axes, respectively.