FIG 3.

Bacterial two-hybrid assay probing EloR against other elongasome proteins. PBP2b, RodA, MreC, MreD, and CozE (CozEa) probed against EloR gave colorless spots of bacteria, complying with no interaction between the two proteins. RodZ, MltG, and YidC2, on the other hand, gave blue spots when probed against EloR, suggesting that an interaction occurs. Positive and negative controls were supplied by the manufacturer (Euromedex). The Jag domain of EloR was tested against the cytosolic domain of MltG with and without the DUF. The interactions between the two domains were lost in the absence of the DUF in the cytosolic part of MltG. This indicates that the Jag domain of EloR interacts with the DUF of MltG.