TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| Strains | ||
| S. pneumoniae | ||
| R704 | R6 derivative; comA::ermAM Eryr | J. P. Claverys |
| RH425 | R704 but streptomycin resistant Eryr Smr | 49 |
| SPH131 | ΔcomA P1::PcomR::comR PcomX::Janus Eryr Kanr | 35 |
| AW407 | ΔcomA P1::PcomR::comR PcomX::eloR-mKate2 Eryr Smr | This work |
| AW408 | ΔcomA P1::PcomR::comR PcomX::jag-mKate2 Eryr Smr | This work |
| AW409 | ΔcomA P1::PcomR::comR PcomX::jag-linker-mKate2 Eryr Smr | This work |
| AW410 | ΔcomA P1::PcomR::comR PcomX::linker-mKate2 Eryr Smr | This work |
| AW420 | ΔcomA P1::PcomR::comR PcomX::eloRK36A-mKate2 Eryr Smr | This work |
| AW424 | ΔcomA P1::PcomR::comR PcomX::eloRK37A-mKate2 Eryr Smr | This work |
| AW425 | ΔcomA P1::PcomR::comR PcomX::eloRF39A-mKate2 Eryr Smr | This work |
| AW426 | ΔcomA P1::PcomR::comR PcomX::eloRL40M-mKate2 Eryr Smr | This work |
| AW453 | ΔcomA P1::PcomR::comR PcomX::eloR-mKate2 ΔstkP::janus Eryr Kmr | This work |
| AW415 | ΔcomA P1::PcomR::comR PcomX::eloR-mKate2 ΔyidC2::janus Eryr Kmr | This work |
| AW417 | ΔcomA P1::PcomR::comR PcomX::eloR-mKate2 ΔrodZ::janus Eryr Kmr | This work |
| AW447 | ΔcomA m(sf)gfp-mltG flag-eloR Eryr Smr | This work |
| AW459 | ΔcomA flag-eloR hlpA-gfp-chloramphenicol Eryr Smr Camr | This work |
| DS515 | ΔcomA m(sf)gfp-mltG Eryr Smr | 19 |
| AW98 | ΔcomA flag-eloR Eryr Smr | 19 |
| MH43 | ΔcomA m(sf)gfp-mltG; ΔyidC2::janus Eryr Kmr | This work |
| SPH472 | ΔcomA m(sf)gfp-mltG; ΔeloR::janus Eryr Kmr | 19 |
| E. coli | ||
| XL1-Blue | Host strain | Agilent Technologies |
| BTH101 | BACTH expression strain; lacks cya | Euromedex |
| Plasmids | ||
| pKT25 | Plasmid used in BACTH analysis | Euromedex |
| pKNT25 | Plasmid used in BACTH analysis | Euromedex |
| pUT18C | Plasmid used in BACTH analysis | Euromedex |
| pUT18 | Plasmid used in BACTH analysis | Euromedex |
| pKNT25-eloR | T25 domain fused to the C terminus of EloR | 19 |
| pKT25-jag | T25 domain fused to the N terminus of the Jag domain of EloR | This work |
| pUT18C-mltG | T18 domain fused to the N terminus of MltG | 19 |
| pUT18C-mltGcyt | T18 domain fused to the N terminus of the cytoplasmic domain of MltG | This work |
| pUT18C-mltGcytΔDUF | T18 domain fused to the N terminus of the cytoplasmic domain of MltG without DUF | This work |
| pUT18C-pbp2b | T18 domain fused to the N terminus of PBP2b | 50 |
| pUT18C-rodA | T18 domain fused to the N terminus of RodA | 50 |
| pUT18C-rodZ | T18 domain fused to the N terminus of RodZ | 19 |
| pUT18C-mreC | T18 domain fused to the N terminus of MreC | 19 |
| pUT18C-mreD | T18 domain fused to the N terminus of MreD | This work |
| pUT18-cozE | T18 domain fused to the C terminus of CozE | 50 |
| pUT18-yidC2 | T18 domain fused to the C terminus of YidC2 | This work |