FIG 7.

Construction of five individual QpH1 ORF knockout vectors and PCR identification of their transformants. (A) Schematic representation of deleted regions (white) of pQGK in five individual QpH1 ORF knockout vectors. The coding sequences of CBUA0036 to CBUA0039a and their direction of transcription are shown by the arrows on the line. (B) The CBUA0006 ORF is absent in transformants of pQGK, pQGK-D1, pQGK-D2, pQGK-D3, and pQGK-D5, suggesting that these transformants are QpH1 deficient. The CBUA0039 ORF is also absent in transformants of pQGK-D2 and pQGK-D3, suggesting that these two transformants contain no plasmid. (C) The CBUA0036 to CBUA0039a ORFs are absent in NMIIpQGK-D2 and NMIIpQGK-D3 but are present in NMIIpQGK.