FIG 6.

Sugar selectivity of VcPA14 and its interactions with C. neogracile. (A) Dextran-based competition binding assay to determine relative affinity of MpPA14 for glucose, GlcNAc, l-fucose, ribose, and GalNAc. (B) Fluorescence levels shown by the C. neogracile cells alone and those treated with FITC-VcPA14 in the absence or presence of 0.5 mM l-fucose. Each bar represents the quantification of normalized average fluorescence from 30 individual diatoms. (C to E) Representative images of diatoms alone (C) and those incubated with FITC-VcPA14 in the absence (D) and presence of 0.5 mM l-fucose (E).