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Neither HSV-1 nor VSVΔG-BHLD entry requires macropinocytosis. C10 (A and B) and CHO-HVEM (C and D) cells were pretreated with macropinocytosis inhibitors cytochalasin D (2 μM), EIPA (25 μM), or NSC23766 (200 μM) and infected with HSV-1 or VSVΔG-BHLD at an MOI of 1. Infectivity was quantitated by flow cytometry at 6 h postinfection. Significance was calculated using a two-tailed Student’s t test with Welch’s correction. *, P < 0.05; **, P < 0.01.
