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. 2021 Mar 2;12(2):e00143-21. doi: 10.1128/mBio.00143-21

FIG 6.

FIG 6

Roles of Rab5 and -7 and Arf6 in HSV-1 and VSVΔG-BHLD entry. The roles of the small GTPases Rab5, Rab7 (A, C, E, and G), and Arf6 (B, D, F, and H) were assessed for HSV-1 (A, B, E, and F) and VSVΔG-BHLD (C, D, G, and H) entry into C10 (A, B, C, and D) and CHO-HVEM (E, F, G, and H) cells. C10 (A and C) and CHO-HVEM (E and G) cells were transfected with either an empty vector control (eGFP or mCherry), eGFP- or mCherry-tagged Rab5 dominant negative (DN), or eGFP- or mCherry-tagged Rab7DN. Cells were infected at an MOI of 1 with either HSV-1 or VSVΔG-BHLD. Entry was assessed by flow cytometry at 6 h postinfection (hpi). The percentage of infected cells was determined by dividing the number of virus+ eGFP/mCherry+ cells by the total number of eGFP/mCherry+ cells. C10 (B and D) and CHO-HVEM cells (F and H) were treated with the Arf6 inhibitor NAV-2729 (25 μM) and infected with either HSV-1 or VSVΔG-BHLD at an MOI of 1. Significance was calculated using a two-tailed Student’s t test with Welch’s correction. *, P < 0.05.