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. 2021 Apr 27;12(2):e00148-21. doi: 10.1128/mBio.00148-21

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Copyright © 2021 Lu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 3 — PfHMGB1 interacts with perinuclear centromeres. (A) Schematic representation of generation of dual-gene-tagging transfectant (PfHMGB1-HA-Ty1::GFP-PfCenH3) by CRISPR-Cas9 with the Pfhmgb1-HA-Ty1 strain as the parent line for transfection. (B) Western blot for PfHMGB1-HA-Ty1 (left) and PfCenH3-N-GFP (right). The aldolase signals were used as the internal control. (C) Co-IFA assay of PfHMGB1-HA-Ty1 (mouse anti-Ty1 antibody, green) and GFP-PfCenH3 (rabbit anti-GFP antibody, red) in the ring or trophozoite-stage parasites of PfHMGB1-HA-Ty1::GFP-PfCenH3 line. Nuclear DNA was stained by DAPI (blue). Bars, 1 μm. (D) IFA assay of GFP-PfCenH3 with anti-GFP antibody in the ring-stage parasites of Pfhmgb1-KO and WT control. Nuclear DNA was stained by DAPI (blue). Bars, 1 μm.