TABLE 3.
Plasmids
| Plasmid | Relevant feature(s) | Reference or source |
|---|---|---|
| pMAD | ori-pE194ts amp erm | 45 |
| pAH9 | ori-pE194 PsarA-mcherry amp erm | 39 |
| pJAB980 | ICE::PIPTG-gfp amp cat | 38 |
| pAC68 | thrC::Pxyl amp erm | Arnaud Chastanet |
| pDR111 | amyE::PIPTG amp spec | David Rudner |
| pRAN332 | Ptet-gfp cat | 54 |
| pEBT13 | Ptet-gfp-rsiP amp erm | 34 |
| pTHE950 | pE194ts ‘thrC lacZ thrB’ cat | 34 |
| pTHE955 | pE194ts ‘thrC PpbpP-lacZ thrB’ cat | This study |
| pEBT2 | ori-pE194ts ΔpbpP amp erm | This study |
| pEBT10 | ori-pE194 PpbpP-pbpP+ amp erm | This study |
| pEBT20 | ori-pE194 Ptet-pbpP+ amp erm | This study |
| pCE693 | ori-pE194 Ptet-pbpPS301A amp erm | This study |
| pCE784 | ICEBs1::PpbpP-pbpP+ amp cat | This study |
| pCE785 | ICEBs1::PpbpP-pbpPS301A amp cat | This study |
| pCE707 | ICEBs1::PIPTG-pbpP+ amp cat | This study |
| pCE726 | ICEBs1::PIPTG-pbpP301A amp cat | This study |
| pCE755 | thrC::Pxyl-pbpP+ amp erm | This study |
| pCE695 | amyE::PIPTG-gfp-rsiP amp spec | This study |
| pCE698 | ICEBs1::PIPTG-gfp-rsiP amp cat | This study |
| pCE697 | ICEBs1::PIPTG amp cat | This study |
| pCE593 | PT7-6×His-rsiP76–275 amp | This study |
| pCE830 | PT7-pbpP35–586 amp | This study |