TABLE 1.

Colocalization of WT and mutant MARCH8, viral glycoproteins, and cellular markers

Viral GPa	r valueb
	MARCH8	MARCH8-CS	+MARCH8		+MARCH8-CS
			Golgi-transferasec	LAMP-1	Golgi-transferase	LAMP-1
VSV-G	0.92 ± 0.08	0.82 ± 0.13	0.91 ± 0.03	0.89 ± 0.06	0.88 ± 0.01	0.16 ± 0.01
VSV-G Tailless	0.85 ± 0.11	0.80 ± 0.09	0.74 ± 0.14	0.31 ± 0.01	0.79 ± 0.15	0.18 ± 0.15
VSV-G K-to-R	0.83 ± 0.01	0.79 ± 0.01	0.90 ± 0.02	0.32 ± 0.04	0.85 ± 0.04	0.14 ± 0.04
HIV-1 Env	0.89 ± 0.13	0.79 ± 0.05	0.86 ± 0.07	0.79 ± 0.04	0.78 ± 0.02	0.18 ± 0.12
HIV-1 EnvCTdel144	0.87 ± 0.12	0.79 ± 0.02	0.79 ± 0.16	0.76 ± 0.04	0.79 ± 0.11	0.19 ± 0.02
EboV-GP	0.78 ± 0.06	0.73 ± 0.12	0.87 ± 0.11	0.67 ± 0.06	0.82 ± 0.08	0.12 ± 0.01
EboV-GP Tailless	0.82 ± 0.08	0.71 ± 0.15	0.81 ± 0.07	0.61 ± 0.21	0.79 ± 0.16	0.15 ± 0.13
SARS-CoV-2 S-FL	0.81 ± 0.01	0.72 ± 0.01	0.68 ± 0.13	0.65 ± 0.16	0.67 ± 0.08	0.20 ± 0.05
SARS-CoV-2 S-FL-K-to-R	0.86 ± 0.11	0.77 ± 0.05	0.67 ± 0.14	0.67 ± 0.21	0.69 ± 0.12	0.18 ± 0.06
SARS-CoV-2 S-Trun	0.89 ± 0.12	0.83 ± 0.03	0.79 ± 0.08	0.85 ± 0.08	0.78 ± 0.06	0.22 ± 0.14
SARS-CoV-2 S-Trun K-to-R	0.92 ± 0.06	0.86 ± 0.13	0.81 ± 0.1	0.81 ± 0.16	0.80 ± 0.21	0.14 ± 0.01

^aViral glycoprotein.

^bPearson correlation coefficient (r) values, means ± standard deviation (SD) using 50 cells per experimental condition. The r values indicate colocalization between MARCH8 and intracellular viral glycoprotein (first two columns), intracellular viral glycoprotein and cellular markers in the presence of WT MARCH8 (two middle columns), and intracellular viral glycoprotein and cellular markers in the presence of MARCH8-CS (right two columns).

^cGolgi-transferase = beta-1,4-galatosyltransferase.