PALM imaging of strains expressing Dendra2-MinD, MinJ-mNeonGreen, and DivIVA-PAmCherry. Representative PALM images of Dendra2-MinD (BHF011), MinJ-mNeonGreen (JB40), and DivIVA-PAmCherry (JB37) expressing cells at different divisional states are shown. Upon formation of a division site, DivIVA, MinJ, and MinD partially relocalize from the poles to the division septum, where they reside after successful cytokinesis. Samples were fixed prior to imaging; every image represents a different cell. Scale bar, 500 nm.