TABLE 5.
Plasmids used in this study
| Plasmid | Characteristics | Reference or source |
|---|---|---|
| pUC18 | lacZα, pMB1 ori, bla (Apr) | 99 |
| pUC18mut | pUC18 with mutated BsaI site in bla | Laboratory collection |
| pDendra2-N | pUC ori, SV40 ori, PCMVIE, aph3-A3 | Evrogen |
| pNCS-mNeonGreen | pUC ori, SV40 ori, bla (Apr) | Allele Biotechnology |
| pUC57-DivIVAd34-mNG | pUC57-BsaI-free, bla (Apr), divIVAΔ34-mNeonGreen | Synthesized by Biocat |
| pHJS105 | amyE integration vector containing Pxyl-msfGFP-MCS, spc bla | 100 |
| pHF01 | pUC18mut-minDup-aad9-Dendra2-minD | This study |
| pHF02 | pUC18mut-minDup-aad9-msfGFP-minD | This study |
| pHF03 | pUC18mut-minJ-msfGFP-aad9-minJdown | This study |
| pHF04 | pUC18mut-minJ-mNG-aad9-minJdown | This study |
| pHF05 | pUC18mut-divIVA-mNG-aad9-divIVAdown | This study |
| pHF06 | pUC18mut-minJ-Dendra2-aad9-minJdown | This study |
| pHF07 | pUC18mut-divIVA-Dendra2-aad9-divIVAdown | This study |
| pHF08 | pUC18mut-divIVAΔ34-mNG-aad9-divIVAdown | This study |
| pHF09 | pUC18mut-minCup-aph3-A3—aad9 | This study |