TABLE 7.
Filter parameters for PALM imaging of the different strainsa
Strain or FP | point spread function (PSF) at half maximum [nm] | No. of photons |
---|---|---|
Dendra2-MinD | 70–160 | 70–250 |
MinJ-mNeonGreen | 70–160 | 70–300 |
DivIVA-PAmCherry | 60–170 | 50–500 |
Filters were chosen according to the fluorophore (FP) behavior in PALM to eliminate background and signal of fluorescent beads from the results.