FIG 4.

Growth inhibition of aspirin on ATCC and CRC isolates of F. nucleatum. Growth of ATCC strains Fn23726 and Fn10953 in response to aspirin as determined by optical density (A) and growth yield (CFU per ml) at 24 h (B). (C) Growth of the CRC F. nucleatum isolates FnCTI-1 and FnCTI-2 in response to aspirin as determined by optical density. (D) Growth yield of FnCTI-1, -2, -3, -5, -6, and -7 in response to 2.5 mM aspirin after 24 h as determined by CFU per ml. All data represent the mean ± SEM for at least 6 cultures. Growth curves were analyzed by two-way repeated measures ANOVA with post hoc Dunnett’s test. Growth was significantly different from sTSB at P values of <0.05 for each of the following strains at the indicated time points and concentrations: Fn23762 (1 mM and 2.5 mM at 7.5 h), Fn10953 (1 mM at 6.5 h and 2.5 mM at 5.5 h), FnCTI-1 (1 mM at 17.5 h and 2.5 mM at 7 h), and FnCTI-2 (1 mM at 9 h and 2.5 mM at 7 h). Analysis of growth yield data was performed by Mann-Whitney test. **, P < 0.01; ***, P < 0.001.