FIG 6.

Fusobacterial abundance in human colonic adenomas of patients who self-reported aspirin use. qPCR was performed on DNA isolated from human colonic adenomas using primers targeting the nusG gene of Fusobacterium spp. and the human PGT gene to normalize for human DNA abundance. Samples were grouped based on patients who reported daily aspirin use and those who reported no aspirin use (white data points). For patients reporting daily aspirin use, gray data points indicate low-dose aspirin (<325 mg daily) and black data points indicate those reporting high-dose aspirin (≥325 mg daily). Data points (circles) represent individual adenomas, and triangles represent adenomas for which Fusobacterium sp. abundance was below the limit of detection and marked at the limit of detection based on the cycle threshold (CT) for hPGT in that individual sample. Bars indicate the mean ± SEM. Analysis was performed by Mann-Whitney test, with * indicating P values of <0.05.