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. 2021 Apr 20;12(2):e00819-21. doi: 10.1128/mBio.00819-21

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Copyright © 2021 Liu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 6 — Immunofluorescence analysis of heart, kidney, and trachea sections from SARS-CoV-2-mCherry-infected K18-hACE2 mice. Tissue cryosections were labeled with Hoechst 33258 dye (nuclei), collagen type IV (basement membrane), and F4/80 (macrophage), and images were acquired by confocal microscopy. Localization of SARS-CoV-2-mCherry in the heart (cross-section) (A), kidney (cross-section) (B), and trachea (longitudinal section) (C) of infected mice at 7 days postinfection. Images were acquired as a z-stack using a 20× (0.95-NA) objective. Arrows show mCherry-positive cells (insets). Scale bars (50 μm) are shown in individual panels.