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Binding analysis of Vag8 mutants by size exclusion chromatography
| Vag8 mutation(s) | Phenotype (C1-INH binding activity) |
|---|---|
| H209A | + |
| A231R | − |
| Y234A | + |
| E237A | + |
| W285A | + |
| H209A Y234A E237A W285A | − |
+, Vag8–C1-INH complex peak was seen; −, proteins eluted separately and no complex peak was observed.
