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. 2021 Mar 9;12(2):e02998-20. doi: 10.1128/mBio.02998-20

FIG 2.

FIG 2

Only B cells trans infect TN. (A) B cells or iDC were pulsed with HIV-1BaL (10−3 MOI) and cocultured with PHA/IL-2-activated purified naive (TN; n = 5) or central memory (TCM; n = 4) CD4+ T cells. Coculture supernatants were tested at the indicated time points for HIV-1 Gag p24 levels by ELISA. Data are mean ± SE. (B) TN were treated with CCL-19, washed, and cocultured with HIV-1BaL (10−3 MOI)-pulsed B cells, iDC, or mature DC (mDC). Coculture supernatants were tested at the indicated time points for HIV-1 Gag p24 by ELISA. Data are mean ± SE, n = 6. (C) Total CD4+ T cells or TN were treated with PHA/IL-2 or CCL-19, pulsed with HIV-1BaL (10−1 MOI), and cultured alone. Culture supernatants were tested at the indicated time points for HIV-1 Gag p24 by ELISA. Data are mean ± SE, n = 4. (D) B cells pulsed with HIV-1 92FR_BX08 (10−3 MOI) were cocultured with CCL-19-treated total CD4+ T cells or TN for up to 12 days. Coculture supernatants were tested at the indicated time points for HIV-1 Gag p24 by ELISA. Data are mean ± SE, n = 3 replicates from 2 donors. (E) iDC pulsed with HIV-1 92FR_BX08 (10−3 MOI) were cocultured with CCL-19-treated total CD4+ T cells or TN for up to 12 days. Coculture supernatants were tested at the indicated time points for HIV-1 Gag p24 by ELISA. Data are mean ± SE, n = 3 replicates from 2 donors. Data were analyzed by one-way ANOVA followed by ad hoc Student’s t test. *, P < 0.05; **, P < 0.005; ***, P < 0.0005; ****, P < 0.0001.