FIG 4.

IL-36γ neutralization reduces lung bacterial burden and the inflammatory response during P. aeruginosa infection in Thbs1^−/− mice. Thbs1^−/− and WT mice were i.t. inoculated with P. aeruginosa at an inoculum of 10⁶ CFU. After 5 hpi, Thbs1^−/− mice were treated with a rabbit anti-mouse IL-36γ neutralizing antibody. Thbs1^−/− and WT mice treated with a rabbit-IgG served as control. At 1 dpi, (A) lung bacterial burden, (B) CXCL-1, (C) CXCL-2, (D) GM-CSF, (E) IL-1β, and (F) IL-17A production in lung tissue homogenates, (G) BALF free NE activity, (H) lung tissue MPO activity, and (I and J) immune cell composition in the BALF were measured. *, P < 0.05 by one-way ANOVA test followed by a post hoc test. Each data point represents an individual mouse; the experiment was performed once without excluding any data. Lines indicate the median.