FIG 5.

TSP-1 tempers neutrophil recruitment and activation and lung cytokine production induced by cleaved IL-36γ. Thbs1^−/− and WT mice were i.t. instilled with 2.5 μg of cleaved IL-36γ (S¹⁸ isoform). At 1 dpi, (A and B) BALF cytospin results showing airspace neutrophil recruitment and BALF macrophages and neutrophils were measured by flow cytometry. (C) BALF free NE and (D) lung tissue MPO were measured in the BALF. In parallel, (E) CXCL-1, (F) CXCL-2, (G) GM-CSF, (H) IL-1β, (I) G-CSF, (J) IL-6, and (K) IL-17A were measured in the lungs. Bar, 100 μm. *, P < 0.05 for single comparisons; the Shapiro-Wilk test was used to assess normal distribution, followed by a Mann-Whitney U test or a parametric t test. A two-way ANOVA test was followed by a post hoc test for multiple comparisons over time. Each data point represents an individual mouse and two independent experiments. Lines indicate the median.