TABLE 1.

Synthetic sequences used in modification of the SEA1 gene

SEA1 region	Synthetic sequencea
SEA1-HA:loxP 5′ recodonized amino acids L318–D450	TTACGCGACAAGCGCGGTAAGTATCACAAGCTTGGAGACTACCAGAACATCGAGAACTACCGCAAGACAGGAGAGCACTCGTTCGACTGCATGAACATGAGCGATATTATGCACAGCAACAAGATGAGTCACGTAAACATTATGGACCACATGATCTACAAGGACAACAATAACATGTCTAAGTTAGTGGACACTATCAACTCACGCGAGAAAGACGTCAAGAACTACGATGACAATTTCGAGTCCTACAACAACTTCTTTAAAAACAACAATGACGAGCAGCACATTTGCTTAGAATACGATGACACCTACAATCTTAAGGACACAGTAAAGAACATCATCGTAGAAGAGGAGCAGTGTGGAAAAGGAGTAGCATGCATTTGCGACAAAAATGAGGAC
SEA1-HA:loxP 3′ recodonized amino acids P1965–I2074	CCAATCAACAAGTTGGCAGTTTCATCTAACTTGGGTCCACCTAGCAGTATTGGCTCTACTGAGATCCAACCAATCCAGAAAAACTTCAATGACTTTAAGATGAACATCAATGTTTATTGTATCCGTATGGAACCACACGAGAAGTATTCGAGCTACAGCCACAAGAACAACCTTGTGGTTTATATCGACAAAGGTGAGAAGATCAACATCATCATCAATATGAGCAAAACCTACGAGAAGGGCGACTTCTTCTATATTCCCCGCTTCAGCAATTTTCAGATTATTAACGACTCCCGCTGCGACTGCGTCCTGTACGTCTGCCCGTTGATT
SEA1 S280A recodonized amino acids E257–N448 with EcoRI site	GAGAACCAGAAGGATATCATCTATCTGAACAACCTGAACAATATCATGATGGACAAGTACAGCAACTGCGCGGACTCGCGCAAGAAAGAGTACTCTCACTTTAACTCCCAAGAATTCTCGTACGACAAGTACAGCATGAAGGATAGGATGTTCCTGAAGAACTTATACATGAAGCAGAACCGCCTGCGCGACAAGCGCGGCAAGTACCATAAGTTAGGAGACTACCAGAACATCGAGAATTACCGCAAGACAGGAGAGCACAGCTTCGACTGCATGAACATGTCCGACATCATGCACTCCAACAAGATGAGTCACGTTAACATAATGGACCATATGATCTACAAGGATAACAACAACATGAGTAAGCTAGTAGACACTATTAACTCACGCGAGAAAGACGTAAAGAACTATGATGACAATTTCGAGAGTTACAACAACTTCTTTAAAAACAATAACGACGAGCAGCATATTTGCTTAGAGTATGATGACACTTACAACTTAAAGGATACTGTAAAGAACATTATCGTGGAGGAGGAGCAGTGCGGTAAAGGAGTTGCTTGCATCTGCGACAAGAAT

^aThe SEA1 S280A codon substitution is shown in boldface italic, and the resulting new EcoRI site is in boldface.