TABLE 2.
PCR primers used in the validation of the integration and excision of gene sequences at the SEA1 locus
| PCR | Primer sequencea |
|---|---|
| SEA1-HA:loxP-3′’ integration (Fig. 1, PCR1) | F, GGACAAACATGAAATGGATTTGAAC |
| R, GCCCATGGCATAGTCCGGGACGTC | |
| SEA1-HA:loxP 5′ integration (Fig. 1, PCR2) | F, GATGGAAAATAAATACCCAAATGA |
| R, CATATATAATAACTTCGTATAATGTATGC | |
| SEA1 wt specific (Fig. 1, PCR3) | F, GATGGAAAATAAATACCCAAATGA |
| R, CATATGATCCATGATATTAACATGGCTC | |
| SEA1-HA:loxP excision (Fig. 1, PCR4) | F, GATGGAAAATAAATACCCAAATGA |
| R, CAATTTATACAAAAATTGTCCTATTTTC | |
| SEA1 bp 135–1940 (Fig. 2) | F, GAATGAAAACGATGGTATATGTGAA |
| R, TCACGTAGCTCATTACTAAGATCCA |
a
F, forward; R, reverse.