FIG 8.

Pdr802 participates in calcineurin signaling. (A and B) Interactions of Pdr802 with upstream regions of the indicated genes. The ratios (log₂) of reads from immunoprecipitated (IP) DNA to input DNA were calculated for 1,000 bp upstream of the first coding nucleotide (+1); shown is the difference in these values between tagged and untagged strains. Black triangles, complete Pdr802 DNA-binding motifs (Fig. S7C); gray triangles, partial motifs. (C) Intracellular calcium measurement by flow cytometry using Fluo-4AM. Each column shows the mean and standard deviation of three biological replicates. ***, P < 0.001, and ****, P < 0.0001, compared to KN99α by one-way ANOVA with a post hoc Dunnett test.