TABLE 3.
Yeast strains used in this study
| Yeast strain | Species | Main characteristics | Source or reference |
|---|---|---|---|
| ER18alpha | Saccharomyces cerevisiae | Haploid segregant of the first-generation bioethanol strain Ethanol Red (Fermentis/Lesaffre) | 83 |
| ER18A | Saccharomyces cerevisiae | Mating type switched ER18alpha | 83 |
| ER18A HPH | Saccharomyces cerevisiae | ER18A with an HPH resistance marker introduced at chrIV at position 867910 (genome version R64-2-1 S288C; SGD), MATa | This study |
| KEA1 | Saccharomyces cerevisiae | Whole-genome transformant of ER18A HPH with DNA of K. marxianus CBS6014 | This study |
| KEA2 | Saccharomyces cerevisiae | Whole-genome transformant of ER18A HPH with DNA of K. marxianus CBS6014 | This study |
| KEA9 | Saccharomyces cerevisiae | Whole-genome transformant of ER18A HPH with DNA of K. marxianus CBS6014 | This study |
| KEA17 | Saccharomyces cerevisiae | Whole-genome transformant of ER18A HPH with DNA of K. marxianus CBS6014 | This study |
| KEA21 | Saccharomyces cerevisiae | Whole-genome transformant of ER18A HPH with DNA of K. marxianus CBS6014 | This study |
| KEA24 | Saccharomyces cerevisiae | Whole-genome transformant of ER18A HPH with DNA of K. marxianus CBS6014 | This study |
| KEA26 | Saccharomyces cerevisiae | Whole-genome transformant of ER18A HPH with DNA of K. marxianus CBS6014 | This study |
| KEA28 | Saccharomyces cerevisiae | Whole-genome transformant of ER18A HPH with DNA of K. marxianus CBS6014 | This study |
| OEA26 | Saccharomyces cerevisiae | Whole-genome transformant of ER18A HPH with DNA of O. polymorpha CBS5032 | This study |
| OEA28 | Saccharomyces cerevisiae | Whole-genome transformant of ER18A HPH with DNA of O. polymorpha CBS5032 | This study |
| OEA34 | Saccharomyces cerevisiae | Whole-genome transformant of ER18A HPH with DNA of O. polymorpha CBS5032 | This study |
| OEA39 | Saccharomyces cerevisiae | Whole-genome transformant of ER18A HPH with DNA of O. polymorpha CBS5032 | This study |
| OEA40 | Saccharomyces cerevisiae | Whole-genome transformant of ER18A HPH with DNA of O. polymorpha CBS5032 | This study |
| OEA41 | Saccharomyces cerevisiae | Whole-genome transformant of ER18A HPH with DNA of O. polymorpha CBS5032 | This study |
| OEA46 | Saccharomyces cerevisiae | Whole-genome transformant of ER18A HPH with DNA of O. polymorpha CBS5032 | This study |
| OEA57 | Saccharomyces cerevisiae | Whole-genome transformant of ER18A HPH with DNA of O. polymorpha CBS5032 | This study |
| KEA17 EAF1ER18A | Saccharomyces cerevisiae | KEA17 EAF1KEA17::EAF1ER18A | This study |
| KEA17 BSC5pER18A | Saccharomyces cerevisiae | KEA17 BSC5pKEA17::BSC5pER18A | This study |
| KEA17 tK(CUU)KER18A | Saccharomyces cerevisiae | KEA17 tK(CUU)KKEA17::tK(CUU)KER18A | This study |
| KEA17 NHA1pER18A | Saccharomyces cerevisiae | KEA17 NHA1pKEA17::NHA1pER18A | This study |
| KEA24 YHK8ER18A | Saccharomyces cerevisiae | KEA24 YHK8KEA24::YHK8ER18A | This study |
| KEA24 RTT10pER18A | Saccharomyces cerevisiae | KEA24 RTT10pKEA24::RTT10pER18A | This study |
| KEA24 HMX1pER18A | Saccharomyces cerevisiae | KEA24 HMX1pKEA24::HMX1pER18A | This study |
| KEA24 EMT2ER18A | Saccharomyces cerevisiae | KEA24 EMT2KEA24::EMT2ER18A | This study |
| KEA24 RPL36BpER18A | Saccharomyces cerevisiae | KEA24 RPL36BpKEA24::RPL36BpER18A | This study |
| OEA28 PIS1ER18A | Saccharomyces cerevisiae | OEA28 PIS1OEA28::PIS1ER18A | This study |
| OEA28 CET1pER18A | Saccharomyces cerevisiae | OEA28 CET1pOEA28::CET1pER18A | This study |
| OEA28 ILV2ER18A | Saccharomyces cerevisiae | OEA28 ILV2OEA28::ILV2ER18A | This study |
| OEA28 MPH1ER18A | Saccharomyces cerevisiae | OEA28 MPH1OEA28::MPH1ER18A | This study |
| OEA28 trt2ER18A | Saccharomyces cerevisiae | OEA28 TRT2OEA28::trt2ER18A | This study |
| ER18A HPH tK(CUU)KKEA17 | Saccharomyces cerevisiae | ER18A HPH tK(CUU)KER18A::tK(CUU)KKEA17 | This study |
| ER18A HPH EMT2KEA24 | Saccharomyces cerevisiae | ER18A HPH EMT2ER18A::EMT2KEA24 | This study |
| ER18A HPH TRT2OEA28 | Saccharomyces cerevisiae | ER18A HPH trt2ER18A::TRT2OEA28 | This study |
| QD47 | Saccharomyces cerevisiae | Diploid; cross of OEA28 and ER18alpha | This study |
| QD48 | Saccharomyces cerevisiae | QD47 TRT2/trt2::NatMX | This study |
| BTC.1D | Saccharomyces cerevisiae | Haploid segregant of ale strain JT 22490; MATα | (84) |
| K11 | Saccharomyces cerevisiae | Acetic acid-tolerant sake brewing strain | Lab strain collection |
| GS1.11-26 | Saccharomyces cerevisiae | Diploid, second-generation bioethanol production strain | (85) |
| CBS5032 | Ogataea polymorpha | Haploid; high-temp growth on solid nutrient plates | CBS-KNAW |
| CBS6014 | Kluyveromyces marxianus | Diploid; high-temp growth on solid nutrient plates | CBS-KNAW |
| CBS7555 | Zygosaccharomyces bailii | Haploid; high acetic acid tolerance and moderate thermotolerance | CBS-KNAW |
| CBS732 | Zygosaccharomyces rouxii | Haploid; highly osmotolerant and moderate thermotolerance | CBS-KNAW |