. 2021 Apr 19;12:646159. doi: 10.3389/fimmu.2021.646159

Table 2.

Primer sequences used for fusion constructions in this study.

Primer	Order	Sequence (5’→3’)	Gene BankAccession No.
huIL-15	Forward	CACCGGTACCAACTGGGTGAATGTAATAAGTGATTTG	NM_172175
huIL-15	Reverse	CGGGATCCAGAAGTGTTGATGAACATTTGGAC	NM_172175
huIgHg2	Forward	CACCGGATCCGCCTCCACCAAGGGCCCAT	AJ250170
huIgHg2	Reverse	GCTCTAGATCATTTACCCGGAGACAGGGAGAGGCTCTTCTGT	AJ250170
mIL-15	Forward	TAGGTACCGGCATTCATGTCTTCATTTTGG	NM_008357
mIL-15	Reverse	TAGGATCCGGACGTGTTGATGAACATTTGG	NM_008357
mIgHg2a	Forward	AGGATCCGCCAAAACAACAG	KC295246.1
mIgHg2a	Reverse	TCTAGAATCATTTACCCGGAGTCC	KC295246.1

The nucleotide sequence for each gene was procured from the Gene Bank (NCBI). The Accession numbers were given for each sequence. The primers having restriction enzyme recognition sequences were designed by using PrimerQuest Tool (Integrated DNA Technologies) and OligoPerfect Designer (Thermo Fisher Scientific). The HPLC purified primers (Sigma Aldrich) were used.