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. 2021 Apr 20;118(17):e2102753118. doi: 10.1073/pnas.2102753118

Fig. 2.

Fig. 2.

Induction of FRIGIDA sectors during embryogenesis and influence on flowering time. (A) Schematic of the 35S:lox-GUS-lox-FRI-GFP construct before and after heatshock. (B) GUS staining in a mosaic plant heat shocked as a 2-d-old seedling. (C) Flowering time assessed by final leaf number in plants that were heat shocked at different stages of embryo development. White bars, plants with FRI-expressing sectors covering all leaves; hatched bars, plants with FRI-expressing sectors and FRI-lacking sectors; black bars, plants with no FRI-expressing sectors. Shaded regions show the range of control plants: Columbia-0 (fri) or plants transformed with an active FRI allele. (D) Leaf number at flowering of 35S:lox-GUS-lox-FRI-GFP plants heat shocked as 2-d-old seedlings for 30 min. Control ranges are shaded as in C.