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. 2021 Apr 21;118(17):e2022294118. doi: 10.1073/pnas.2022294118

Fig. 6.

Fig. 6.

Phylogenetic relationship of aromatic O-PTs in the UbiA superfamily from Rutaceae and Apiaceae. A neighbor-joining phylogenetic tree of UbiA proteins. The tree was constructed with 1,000 bootstrap tests based on a ClustalW multiple alignment of UbiA proteins, including CpPT1, CmiPT1a/b, and AkPT1. Bootstrap values are shown for nodes separating clades and for nodes between C-PTs and O-PTs in Rutaceae and Apiaceae. The bar represents an amino acid substitution rate per site of 0.20. The clades of primary metabolism-related proteins are marked with gray circles. The clades of specialized metabolism-related PTs are highlighted with differently colored circles depending on their possible ancestors, with VTE2-1-, VTE2-2-, and PPT-related clades indicated in orange, green, and blue, respectively, together with their aromatic substrates at the family scale. The PTs of Rutaceae and Apiaceae isolated in this study are highlighted in red and bold. The plant species and the accession numbers of the input sequences are provided in SI Appendix, Table S5.