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. 2021 Apr 19;118(17):e2021596118. doi: 10.1073/pnas.2021596118

Fig. 2.

Fig. 2.

GPR182 binds the chemokines CXCL10, CXCL12, and CXCL13. (A) Binding of the fluorescently labeled human chemokine, CXCL12-AF647, to human GPR182, expressed by HEK293T cells (n = 3). (B) Competition of hCXCL12-AF647 and unlabeled hCXCL10 and hCXCL12β for binding to human GPR182 (n = 3). (C) Binding of hCXCL10-AF647 to hGPR182 (n = 3). (D) Effect of various chemokines (120 nM) on binding of hCXCL10-AF647 to hGPR182. (E) Competition of hCXCL10-AF647 and indicated unlabeled human chemokines for binding to GPR182. (F) Competition of human CXCL10-AF647 and various unlabeled murine chemokines for binding to mGPR182. Individual experiments were performed in triplicates; shown is one, representative of four (C), two (D) and three (E and F) independently performed experiments. Shown are mean values ± SEM of one experiment.