Fig. 6.

Phosphorylation of GSP-1 at S2 partially rescues the mislocalization of H3pT3 observed in him-17 mutants. (A) Histograms indicate the percentage of bivalents at diakinesis with H3pT3 localization restricted to the short arm, mislocalized to both long and short arms, faint but on both long and short arms, faint but only on short arms, or missing on the chromosomes in the indicated genotypes. Chromosome abnormalities, consisting of aggregates and presence of DNA fragments, were observed in gsp-1pd him-17(RNAi) + IR. At least 100 bivalents were scored for each genotype from between 12 to 65 animals from three biological replicates. (B) Histogram indicates the percentage of bivalents at diakinesis with LAB-1 localization observed restricted to the long arm, mislocalized to both long and short arms, faint on both long and short arms, faint only on the short arm, or missing from chromosomes. Chromosome abnormalities, consisting of aggregates and DNA fragments, were observed in gsp-1pd him-17(RNAi) + IR. At least 72 bivalents were scored for each genotype from three biological replicates. ****P < 0.0001 two-tailed Fisher’s exact test. For complete/additional statistical analysis, see Dataset S1.