(A–D) Echocardiographic analysis of cardiac function in NLC and TgGRK5nt mice 8 weeks post- TAC or sham operation. Ejection fraction % (A), fractional shortening % (B), posterior wall thickness (C), and left-ventricular internal diameter (LVID) (D). (E and F) Heart weight (E) and lung weight (F) indexed to tibia length (HW/TL, LW/TL). N=10 NLC Sham mice, N=11 NLC TAC mice, N=7 TgGRK5nt Sham mice, N=7 TgGRK5nt TAC mice for (A) to (F). (* p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001, two-way ANOVA with Tukey’s post-hoc testing for multiple comparisons).