ISRCTN13374800.

Study name	Alternate day fasting as an intervention to improve body composition and blood biochemical markers in overweight patients
Methods	Randomised controlled trial
Participants	Key inclusion & exclusion criteria Inclusion criteria 1. 20‐55 years of age 2. BMI > 25 kg/m2 3. Previous sedentary lifestyle 4. Absence of diabetes, hypertension and cardiovascular diseases 5. No smoking Exclusion criteria 1. Previously undergone bariatric surgery
Interventions	Alternate day fasting (ADF) group: participants in this group have their calorie intake limited to 25% of the baseline energy needs on the fasting days and this is to be ingested within a 12‐hour period and divided into six small meals. On the feeding days, ad libitum food intake was permitted over a 12‐hour period. On both days, ingestion of food is not permitted in the remaining hours during which the participants are awake. On the feeding days, there is no restriction on calorie intake, but the participants are required to record the specific consumed foods in a food diary. Carloric restriction (CR) group: participants in this group have a hypocaloric diet that is restricted to 75% of the estimated calorie expenditure per day throughout the intervention period.
Outcomes	1. Mean body weight loss is measured using the BodPod and InBody770 at baseline and 4 weeks 2. Fat loss is measured using the BodPod and InBody770 at baseline and 4 weeks 3. Lean mass loss is measured using the BodPod and InBody770 at baseline and 4 weeks 4. Carbohydrate burning is measured using indirect calorimetry at baseline and 4 weeks 5. BMR (Basal Metabolic Rate) is measured using Indirect calorimetry (Spirostik‐REE) at baseline and 4 weeks 1. Body water is measured using InBody770 at baseline and 4 weeks 2. Food patterns is measured using a Food Reminder Form that was delivered for all subjects at baseline and 4 weeks
Starting date	08/08/2017
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