FIG 3.

The binding of the bispecific antibodies to gB and pentamer by a BLI assay. Protein A biosensors were used to capture the indicated antibodies. The biosensors loaded with antibodies were then dipped in titrated (A) gB or (B) pentamer solutions. One loaded biosensor was dipped in buffer-only solution to serve as a reference. The data were reference subtracted and then fitted to a 1:1 binding model using the Octet Data Analysis software. (C) The kinetic constants and fitting parameters for gB and pentamer binding to the antibodies.