FIG 5.

HHA and UDA reduce membrane mobility in HA-expressing cells. HeLa cells expressing B/Netherlands/537/2005-HA were labeled with Alexa Fluor 488 and treated with HHA or UDA, followed by FRAP analysis. Data shown are the mean ± SD of three independent experiments. Statistical analysis showed a significant decrease for the curves for HHA at 40 nM (P < 0.001), UDA at 240 nM (P < 0.001), and UDA at 24 nM (P = 0.0051), compared to the no-compound control (Mann-Whitney test, two-tailed; confidence level, 99%).