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. 2021 Feb 17;65(3):e01771-20. doi: 10.1128/AAC.01771-20

TABLE 5.

Gradient dilution for MIC determinations

Dilution step Concentrationa
1 C1
2 C2
3 C1/2
4 C2/2
5 C1/4
6 C2/4
7 C1/8
8 C2/8
9 C1/16
10 C2/16
11 C1/32
12 0
a

C2 equals (C1 + C1/2)/2 equals 3C1/4 or C2 is a special concentration (C1 < C2 < C1/2) from pilot experiments.