Glucose resensitization mechanism is independent of growth resumption. Stationary-phase cultures were washed with, resuspended in, and further incubated in saline for 1 h before addition of water (A), 10 mM glucose (B), 9.5 mM NH4Cl (C), 1.32 mM K2HPO4 (D), or 0.286 mM MgSO4 (E). Concentrations were based on those found in minimal M9 medium (53). Following treatments, cultures were incubated for 7 h and CFUs were determined at the indicated time points. *, P < 0.05 (t test), comparing glucose with glucose plus NIT. Data represent at least three biological replicates. Each data point was denoted as mean ± SE.