TABLE 1.
Selected and labeled primers for single nuclear polymorphisms detection in the pfdhfr genea
| Primer nameb | Sequence and modification (5′→3′)c | Descriptiond |
|---|---|---|
| Dig-Pfdhfr-N51I_Rev | Dig-GCTTTCCCAGCTTGTTCTTCCC | Common primer |
| Bio-Pfdhfr-N51_Fwd_WT (W') | Bio-AGGAGTATTACCATGGAAATGT*A*A | Allele-specific primer |
| Bio-Pfdhfr-51I_Fwd_Mut (M') | Bio-AGGAGTATTACCATGGAAATGT*A*T | |
| Dig-Pfdhfr-59_108_Fwd | Dig-GATGGAACAAGTCTGCGACGT | Common primer |
| Bio-Pfdhfr-C59_Rev4_WT (W4') | Bio-TCATTCACATATGTTGTAACTGCT*C*A | Allele-specific primer |
| Bio-Pfdhfr-59R_Rev4_Mut (M4') | Bio-TCATTCACATATGTTGTAACTGCT*C*G | |
| Bio-Pfdhfr-S108_Rev3_WT (W3') | Bio-TTTTGGAATGCTTTCCCT*G*C | Allele-specific primer |
| Bio-Pfdhfr-108N_Rev3_Mut (M3') | Bio-TTTTGGAATGCTTTCCCT*G*T |
a
Gene identifier PF3D7_0417200.
b
Bio, biotin; Dig, digoxin; Fwd, forward; Rev, reverse; WT, wild type; Mut, mutant.
c
Artificial mismatches are in boldface font. *, the location of phosphorothioate modification.
d
The same common primer utilized in allele-specific amplification of C59R and S108N of Pfdhfr.