FIG 1.

Percent growth of four classes of antifungal agents employing resazurin (RZ) and XTT assays in testing Madurella mycetomatis CBS131320 isolate. (A) Percent growth obtained with XTT and resazurin for amphotericin B. At 4 µg/ml, the percent growth for both resazurin and XTT is below the 20% cutoff line. This is the MIC for this particular isolate. (B) Percent growth for itraconazole. At 0.125 µg/ml, the percent growth for both resazurin and XTT is below the 20% cutoff line. This is the MIC for itraconazole for this particular isolate. (C, D) Percent growth for terbinafine and micafungin. For both drugs, the growth percentages obtained with XTT and resazurin were never below the 20% cutoff line, and no MIC was obtained at the concentrations tested.