TABLE 2.
Characteristics of transconjugants
| Strain | Cip concn (µg/liter) | na | Resistanceb |
MLSTc | Resistance genes (% identity) | Resistance mutations | Virulence gene(s) | Plasmid replicons | Transfer event | |
|---|---|---|---|---|---|---|---|---|---|---|
| Cip | Str | |||||||||
| SP132 | S/R | R | ST409 | ctx-m-1, mdf(A) | wt or gyrA S83L | gad | IncI1 (100%) | |||
| SP163 | R | S | ST359 | tet(A), mdf(A) | gyrA S83L, D87N, parC S80I | cba, cma, iroN, iss, lpfA, mchF | IncFIB (AP001918), IncFIB (pLF82), IncFIC (FII) | |||
| Transconjugants | 0 | 4 | R | S | ST359 | tet(A), ctx-m-1, mdf(A) | gyrA S83L, D87N, parC S80I | cba, cma, gad, iroN, iss, lpfA, mchF | IncI1, IncFIB (AP001918), IncFIB (pLF82), IncFIC (FII) | SP163 recipient; SP132 donor |
| 5 | S | S | ST11511 | tet(A) (99%), ctx-m-1 | parC S57T, parE I355T | gad | IncI1, p0111 | Endogenous Tetr recipient; SP132 donor | ||
| 1 | 5 | S | S | ST11511 | tet(A) (99%), ctx-m-1 | parC S57T, parE I355T | gad | IncI1, p0111 | Endogenous Tetr recipient; SP132 donor | |
| 17 | S | S | ST48 | tet(A), ctx-m-1, mdf(A), aadA5, sul2, dfrA17 | No mutations | gad | IncI1, IncFIB (AP001918), IncFIB (pLF82), IncFII (pHN7A8), p0111 | Endogenous Tetr recipient; SP132 donor | ||
| 12 | R | S | ST359 | tet(A), ctx-m-1, mdf(A) | gyrA S83L, D87N, parC S80I | cba, cma, gad, iroN, iss, lpfA, mchF | IncI1, IncFIB (AP001918), IncFIB (pLF82), IncFIC (FII) | SP163 recipient; SP132 donor | ||
| 5 | 5 | S | S | ST165 | tet(A), ctx-m-1, mdf(A) | No mutations | gad | IncI1 | Endogenous Tetr recipient; SP132 donor | |
| 2 | R | R | ST409 | tet(B), ctx-m-1, mdf(A) | gyrA S83L, rpsL K43R | gad | IncI1, IncHI2, IncHI2A | SP132 recipient; endogenous Tetr donor | ||
| 20 | 2 | S | S | ST165 | tet(A), ctx-m-1, mdf(A) | No mutations | gad | IncI1 | Endogenous Tetr recipient; SP132 donor | |
a
n = number of transconjugants.
b
Cip, ciprofloxacin; Str, streptomycin; S, sensitive; R, resistant according to EUCAST breakpoints.
c
MLST is reported according to the Achtman scheme. Identity to the reference is given when <100%.