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. 2021 Apr 20;118(17):e2024258118. doi: 10.1073/pnas.2024258118

Fig. 6.

Fig. 6.

DDX11 is complementary with BRCA2 in facilitating DNA repair and genome stability (A) Quantification and representative micrographs of γ-H2AX and 53BP1 foci in U2OS Ctrl and DDX11 KO cells after 72 h of siCtrl and siBRCA2 transfection. (Scale bar, 10 μm.) n = 2. Statistical analysis was performed using Student’s t test. Error bars show average ± SD. (B) Quantification and representative micrographs of micronuclei in U2OS Ctrl and DDX11 KO cells after 72 h of indicated siRNAs transfection, n = 2. Statistical analysis was performed using Student’s t test. Error bars show average ± SEM. (C) Cell viability assay of PEO1 (S) and C4-2 (R) cells transfected with siCtrl and siDDX11. Cells were treated with olaparib with the indicated drug concentrations for 5 to 6 d (n = 3). Cell viability was determined using crystal violet staining. (Top Right) Schematic representations of BRCA2 mutations and (Bottom Right) Western blot of DDX11 and BRCA2 variants. Error bars show average ± SEM. (D) Cell viability assay of Capan-1 (S) and Capan-1 (R) cells transfected with indicated siRNAs. Cells were treated with olaparib with the indicated drug concentrations for 5 to 6 d (n = 3). Cell viability was determined using crystal violet staining. (Top Right) Schematic representations of BRCA2 mutations and (Bottom Right) Western blot of DDX11 and BRCA2 variants. Error bars show average ± SEM.