Table 1. Antibodies used in flow cytometry.
| Antibody | Species | Clone | Fluorochrome | Labeling strategy | Isotype | Concentration (mg/mL) | Working dilution |
|---|---|---|---|---|---|---|---|
| 7-AAD | NA | NA | 7-AAD | Dye | NA | 0.1 | 1/400 |
| granulocyte | mouse anti-pig | 6D10 | FITC | Directly conjugated | IgG2a | 0.1 | Neat |
| CD45RA | mouse anti-pig | MIL13 | FITC | Directly conjugated | IgG1 | 0.1 | Neat |
| CD4alpha | mouse anti-pig | MIL17 | FITC | Directly conjugated | IgG2b | 0.1 | Neat |
| IgM | goat anti-pig | AAI48 | FITC | Directly conjugated | IgM | 1 | 1/100 |
| CD5 | mouse anti-pig | 1H6/8 | FITC | Directly conjugated | IgG2a | 0.1 | Neat |
| CD14 | mouse anti-pig | MIL2 | FITC | Directly conjugated | IgG2b | 0.1 | 10-Jan |
| CD79a | mouse anti-human | HM57 | RPE | Directly conjugated | IgG1 | Not determined | 10-Jan |
| CD4alpha | mouse anti-pig | MIL17 | RPE | Directly conjugated | IgG2b | Not determined | 1/100 |
| CD8alpha | mouse anti-pig | MIL12 | RPE | Directly conjugated | IgG2a | Not determined | 1/100 |
| CD45RA | mouse anti-pig | MIL13 | RPE | Directly conjugated | IgG1 | Not determined | 10-Jan |
| CD16 | mouse anti-pig | G7 | RPE | Directly conjugated | IgG1 | Not determined | 10-Jan |
| CD3 | mouse anti-pig | PPT3 | RPE-Cy7a | Secondary antibodya | IgG1 | 0.1 | 10-Jan |
| SWC7 or WC4 | mouse anti-bovine | CC55 | RPE-Cy7a | Secondary antibodya | IgG1 | 0.1 | Neat |
| macrophages | mouse anti-pig | BA4D5 | APCb | Secondary antibodyb | IgG2b | 0.1 | Neat |
| SWC2 or CD27 | mouse anti-pig | B30C7 | APC | Directly conjugated | IgG1 | Not determined | 10-Jan |
| CD335 | mouse anti-pig | VIV-KM1 | APC | Directly conjugated | IgG1 | Not determined | 1/100 |
| IgG1 isotype control | mouse | NA | FITC | Directly conjugated | IgG1 | 0.1 | 10-Jan |
| IgG2a isotype control | mouse | NA | FITC | Directly conjugated | IgG2a | 0.1 | 10-Jan |
| IgG2b isotype control | mouse | NA | FITC | Directly conjugated | IgG2b | 0.1 | 10-Jan |
| IgG1 isotype control | mouse | NA | RPE | Directly conjugated | IgG1 | Not determined | 10-Jan |
| IgG2a isotype control | mouse | NA | RPE | Directly conjugated | IgG2a | Not determined | 10-Jan |
| IgG2b isotype control | mouse | NA | RPE | Directly conjugated | IgG2b | Not determined | 10-Jan |
| IgG1 isotype control | mouse | NA | APC | Directly conjugated | IgG1 | Not determined | 10-Jan |
| IgG1 isotype control | mouse anti-pig | NA | RPE-CY7a | Secondary antibodya | IgG1 | 0.1 | Neat |
a MAb dilution following PE-Cy7 labeling (Serotec).
b MAb dilution following APC labeling (Serotec).
NA, not applicate.