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. 2021 Mar 10;95(7):e02141-20. doi: 10.1128/JVI.02141-20

TABLE 1.

Primers used for PCR-based mutagenesis

Primer name Sequence
Forward mutI NF-κB GGACTTTCCGCTGTCTACTTTCCAGG
Reverse mutI NF-κB CCTGGAAAGTAGACAGCGGAAAGTCC
Forward mutII NF-κB GCTTTCTACAATCTACTTTCCGCTGG
Reverse mutII NF-κB CCAGCGGAAAGTAGATTGTAGAAAGC
Forward mutI&II NF-κB GCTTTCTACAATCTACTTTCCGCTGTCTACTTTCCAGG
Reverse mutI&II NF-κB CCTGGAAAGTAGACAGCGGAAAGTAGATTGTAGAAAGC
Forward delNF-κB GCTGACATCGAGCTTTCTACAAAGGGAGGTGTGGCCTGGGCGGG
Reverse delNF-κB CCCGCCCAGGCCACACCTCCCTTTGTAGAAAGCTCGATGTCAGC
Forward mutISRE GCCCGAACAGGGACTTGCCCGCGCCCGTAAAGCCAGAGGAGATC
Reverse mutISRE GATCTCCTCTGGCTTTACGGGCGCGGGCAAGTCCCTGTTCGGGC