FIG 8.

Histology and immunohistochemical staining of the uninfected control, rSD-WT-, rSD-H238A-, and rSD-Y334A-infected chicken tissues. Chickens were euthanized on day 7. Images show representative histological slides of the trachea, lungs, and kidneys. Images were stained with hematoxylin and eosin (A) and mouse anti-IBV-N antibody (B). In panel A, the black arrow indicates extensive dropout, degeneration, and necrosis of the ciliated epithelial cells; the black triangle indicates hemorrhaging in the bronchial lumen; the open triangles indicate exfoliated fibroblasts and mucus generated from the accessory bronchial lumen; the open arrow indicates renal tubulointerstitial lymphocyte infiltration.