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. 2021 Mar 10;95(7):e01968-20. doi: 10.1128/JVI.01968-20

TABLE 2.

Second experimental infection

Animal Species Age category (age [mo]) Inoculation route Virus, dose (FFFU) Sample collection time point (day postinoculation) Result(s)a
RT-PCR (brain, lung, blood) Blood smear Histology IHC
2.1 P. regius Juvenile (∼2) Trachea Mock (PBS) 118 Neg, Neg, Neg Neg Neg Neg
2.2 P. regius Juvenile (∼2) Trachea Mock (PBS) 22 Neg, Neg, Neg Neg Neg Neg
2.3 P. regius Juvenile (∼2) Trachea UHV (UHV-1 and ABV-1), 50,000 22 Neg, UHV-1 and ABV-1, Neg Neg Neg Neg
2.4 P. regius Juvenile (∼2) Trachea UHV (UHV-1 and ABV-1), 50,000 69 Neg, Neg, Neg Neg Neg Neg
2.5 P. regius Juvenile (∼2) Trachea UHV (UHV-1 and ABV-1), 50,000 30 ABV-1, ABV-1, ABV-1 Neg Neg Pos
2.6 P. regius Juvenile (∼2) Trachea UHV (UHV-1 and ABV-1), 50,000 117 Neg, ABV-1, Neg Neg Neg Neg
2.7 P. regius Juvenile (∼2) Trachea UGV-1, 50,000 22 UGV-1, UGV-1, UGV-1 Neg Neg Neg
2.8 P. regius Juvenile (∼2) Trachea UGV-1, 50,000 117 Neg, UGV-1, Neg Neg Neg Neg
2.9 B. constrictor Juvenile (∼4) None Cohousing with animal 2.4 117 Neg, Neg, Neg Neg Neg Neg
2.10 B. constrictor Juvenile (∼4) None Cohousing with animal 2.8 117 Neg, Neg, Neg Neg Neg Neg
a

Pos, positive.