. 2021 Mar 10;95(7):e01968-20. doi: 10.1128/JVI.01968-20

TABLE 4.

Antibody responses in experimentally compared to naturally reptarenavirus-infected boas^a

Animal	Virus(es) used for inoculation or immunization	ELISA titer (UGV-1 lysate)		Blood smear score	Neut. titer					RT-PCR result(s)
Animal	Virus(es) used for inoculation or immunization	IgY	IgM	Blood smear score	UGV-1	UHV-1	ABV-1	TSMV-2	S5-like	RT-PCR result(s)
3.1 0-bleed		0.151	0.052	0	0	0	0	ND	ND	Neg
3.1. final		0.201	0.058	0	0	0	0	ND	ND	Neg
3.2 0-bleed		0.199	0.067	0	0	0	0	ND	ND	Neg
3.2 final		0.109	0.026	0	0	0	0	ND	ND	Neg
3.3 postimm.	Immunized with recombinant UHV-1 NP	3.227	0.069	0	ND	ND	ND	ND	ND	Neg
3.3 final	UHV-1, ABV-1, later UGV-1	3.311	0.109	0	250	150	75	ND	ND	UGV-1
3.4 postimm.	Immunized with inactivated UHV (UHV-1 and ABV-1)	2.838	0.059	0	ND	ND	ND	ND	ND	Neg
3.4 final	UHV-1, ABV-1, later UGV-1	3.092	0.109	0	150	100	75	ND	ND	UGV-1
3.5 postimm.	Immunized with inactivated UHV (UHV-1 and ABV-1)	1.303	0.080	0	ND	ND	ND	ND	ND	Neg
3.5 final	UHV-1 and ABV-1	1.901	0.045	0	500	500	200	ND	ND	UHV-1, ABV-1
3.6 postimm.	Immunized with inactivated UHV (UHV-1 and ABV-1)	3.212	0.026	0	ND	ND	ND	ND	ND	Neg
3.6 final	UHV-1 and ABV-1	3.103	0.041	0	200	400	50	ND	ND	Neg
3.7 final	UHV-1 and ABV-1	3.018	0.081	0	200	150	400	ND	ND	ABV-1
3.8 final	UHV-1 and ABV-1	3.177	0.066	0	400	0	800	ND	ND	ABV-1
3.9 final	UHV-1 and ABV-1	3.177	0.042	0	400	200	800	ND	ND	ABV-1
3.10 final	UHV-1 and ABV-1	2.910	0.039	0	400	200	50	ND	ND	ABV-1
3.11 final	UHV-1, ABV-1, and UGV-1	3.267	0.059	0	400	50	0	ND	ND	UGV-1
3.12 final	UHV-1, ABV-1, and UGV-1	3.319	0.060	0	400	400	400	ND	ND	UGV-1
3.13 final	UGV-1	3.372	0.262	0	400	50	400	ND	ND	UGV-1
3.14 final	UGV-1	3.370	0.049	0	400	1,600	200	ND	ND	UGV-1
Nat. inf. 1		3.042	0.061	0	200			0	6,400	UGV, TSMV-2
Nat. inf. 2		1.839	0.085	0	1,000			50	3,200	TSMV-2
Nat. inf. 3		1.186	0.047	0	500			400	3,400	S5, TSMV-2
Nat. inf. 4		0.045	0.008	0	700			6,400	6,400	Neg
Nat. inf. 5		0.150	0.089	2	90			100	800	UGV, S5, TSMV-2
Nat. inf. 6		0.621	0.045	0	800			350	1,600	S5
Nat. inf. 7		0.222	0.116	2	450			50	350	UGV, S5
Nat. inf. 8		1.914	0.026	0	750			400	3,500	Neg
Nat. inf. 9		0.161	0.021	1	400			300	1,600	UGV, S5
Nat. inf. 10		2.348	0.122	0	400			0	2,200	S5
Nat. inf. 11		2.270	0.257	0	600			800	750	Neg
Nat. inf. 12		0.054	0.018	3	800			1,700	2,400	UGV, S5
Nat. inf. 13		1.546	0.101	0	200			0	300	UGV, TSMV-2
Nat. inf. 14		0.085	0.012	2	1,700			50	1,200	UGV, S5, TSMV-2
Nat. inf. 15		1.828	0.262	1	1,700			300	1,200	UGV
Nat. inf. 16		1.588	0.132	0	800			75	3,700	UGV, S5, TSMV-2
Nat. inf. 17		1.049	0.416	3	1,600			0	6,400	UGV, S5, TSMV-2
Nat. inf. 18		0.699	0.015	1	300			250	6,000	UGV, S5, TSMV-2
Nat. inf. 19		0.217	0.017	0	250			250	2,800	S5, TSMV-2
Nat. inf. 20		0.034	0.060	0	200			200	300	S5, TSMV-2
Nat. inf. 21		1.581	0.078	0	400			400	450	S5, TSMV-2
Nat. inf. 22		0.020	0.045	1	400			250	1,700	UGV, S5, TSMV-2
Nat. inf. 23		1.813	0.054	0	200			200	1,200	UGV, S5, TSMV-2
Nat. inf. 24		0.983	0.706	1	75			100	1,700	S5, TSMV-2

Abbreviations: 0-bleed, blood sample collected before initiation of the experimental work; final, blood sample collected during necropsy; postimm., blood sample collected following immunizations and prior to virus challenge; Nat. inf., naturally infected animal; ND, not determined. Blood smears were scored on a scale of 0 to 3, where a score of 0 is no IBs, 1 is small and/or rare IBs, 2 is medium-sized and/or occasional IBs, and 3 is large and/or numerous IBs.