FIG 4.

shRNA-mediated silencing of dynein cofactors in the SK-N-SH cell line. (A) Puromycin toxicity assay on SK-N-SH cells. A range of different concentrations (0 to 10 μg/ml) of puromycin was used to determine the appropriate amount of puromycin that is toxic to SK-N-SH cells. ***, P < 0.001 versus the no-drug (0 μg/ml)-treated control. Statistical comparison was conducted by GraphPad Prism software using ANOVA with a post hoc t test with Bonferroni adjustment. Error bars represent 95% confidence intervals about the means. (B) Lentivirus shRNA (human)-mediated silencing in SK-N-SH cells. The left lanes in each panel show SK-N-SH lysate treated with control shRNA, and the right lanes show lysates treated with shRNA targeted against motor accessory proteins BICD2, EB1, and the dynactin complex (DCTN2 and DCTN3), respectively.