FIG 5.

Role of motor accessory proteins in HSV-1 intracellular transport. shRNA-treated SK-N-SH cells were synchronously infected with HSV-1 (McKrae) at an MOI of 20 for 30 min, 3 h, and 5 h at 37°C. Control-shRNA-treated SK-N-SH shows the presence of VP5 (ICP5) in the nucleus at 5 hpi (A). (B) Side-by-side comparison between control shRNA and shRNA against motor accessory protein-treated SK-N-SH cells infected with HSV-1 (McKrae) at an MOI of 20 for 5 h. The 8-well chamber slide was fixed with formalin, permeabilized with methanol, and prepared for fluorescence microscopy. Antibody against VP5 (ICP5) (red) was used, and nuclei were stained with DAPI (blue). Capsids colocalized in the nuclei are visible as purple (red plus blue). Magnification of 60× with oil immersion.