FIG 7.

Interactions between HSV-1 capsid/tegument proteins and cellular motor and accessory proteins. (A) Two-way immunoprecipitations (IP) showing UL37 and dynein intermediate-chain (DIC) interaction in HSV-1 (McKrae)-infected SK-N-SH lysates at 2 h postinfection at an MOI of 10. (B) Immunoprecipitation assay showing potential interactions between VP5 and DCTN1, VP5 and p62, and VP5 and EB1 and no interaction between VP5 and DIC proteins. SK-N-SH cells were infected with HSV-1 (McKrae) at an MOI of 10 for an hour, and lysates were prepared with NP-40 lysis buffer with protease inhibitor cocktail. The left lane represents SK-N-SH lysates (control), and the right lane represents lysates immunoprecipitated for HSV-1 VP5 and subsequently probed for dynactin complex (DCTN1 [p150], p62 [DCTN4]), EB1, and DIC.