FIG 1.

Generation and quality control of viral miRNA mutant- and WT-infected TIVE cells. (A) KSHV miRNA locus showing the cluster of 10 miRNAs and 2 in the K12 open reading frame. The -Δall mutant refers to a virus that has the entire cluster deleted plus the two in K12 changed without affecting the K12 ORF. (B) qPCR analysis of episome copy number. Genomic DNA from infected TIVE cells was quantified using LANA-specific primers and compared to a standard curve. (C) RNA-seq-based quantification of LANA expression levels normalized to GAPDH.